1 25 oh 2 d3  (MedChemExpress)

Journal: Nutrients

Article Title: Bone Mineral Density and the Risk of Type-2 Diabetes in Postmenopausal Women: rs4988235 Polymorphism Associated with Lactose Intolerance Effects

doi: 10.3390/nu16173002

Figure Lengend Snippet: Demographic, clinical, and biochemical characteristics of the study group.

Article Snippet: After centrifugation of the serum, a two-day column extraction was performed, followed by determination of vitamin-D3 concentration with a 25–OH–Vitamin D ELISA kit (Immundiagnostik, Bensheim, Germany).

Techniques: Medications

Journal: BioMed Research International

Article Title: In Vivo Determination of Vitamin D Function Using Transgenic Mice Carrying a Human Osteocalcin Luciferase Reporter Gene

doi: 10.1155/2013/895706

Figure Lengend Snippet: Constructs for the generation of the transgenic mice. (a) Schematic map of the transgenes used in the study. pOC-Luc is composed of a 10-kb human osteocalcin enhancer/promoter sequence, with 60 bp of the 5′-untranslated sequence (white box), a luciferase gene (black box), an SV40 late polyadenylation signal (striped box), and an insulator sequence (gray box). (b) Enhanced expression of pOC-Luc by 1 α ,25(OH) 2 D3 treatment. MG-63 and HeLa cells transfected with pOC-Luc or pΔOC-Luc were incubated with 1 nM previtamin D3, 1 α ,25(OH) 2 D3, previtamin D2, 1 α ,25(OH) 2 D2, or vehicle, and then subjected to luciferase reporter assays. The y -axis shows the relative luciferase activity, representing the firefly luciferase (FL) activity from the reporter plasmid normalized by the Renilla luciferase (RL) activity from the control vector. Statistical analysis was performed by Student's t -test; * P < 0.005 relative to vehicle.

Article Snippet: Dimethylsulfoxide (DMSO) as the vehicle or 1 nM of previtamin D2 (Sigma-Aldrich Co., St. Louis, MO, USA), previtamin D3 (Sigma-Aldrich), 1 α ,25(OH) 2 D2 (Sigma-Aldrich), and 1 nM 1 α ,25(OH) 2 D3 (Merck KGaA, Darmstadt, Germany) in DMSO (0.01% final DMSO concentration) were added at the same time as the transfection.

Techniques: Construct, Transgenic Assay, Sequencing, Luciferase, Expressing, Transfection, Incubation, Activity Assay, Plasmid Preparation

Journal: BioMed Research International

Article Title: In Vivo Determination of Vitamin D Function Using Transgenic Mice Carrying a Human Osteocalcin Luciferase Reporter Gene

doi: 10.1155/2013/895706

Figure Lengend Snippet: Regulation of the human osteocalcin enhancer/promoter by vitamins D3 and D2 in vivo . (a) Enhanced expression of the OC-Luc transgene by 1 α ,25(OH) 2 D3 treatment. OC-Luc Tg mice were given a single oral administration of previtamin D3, 1 α ,25(OH) 2 D3, or vehicle. In vivo bioluminescence imaging was performed at 6 h after the administration. (b) Response of the OC-Luc transgene to 1 α ,25(OH) 2 D2 treatment. OC-Luc Tg mice were given a single oral administration of previtamin D2, 1 α ,25(OH) 2 D2, or vehicle. In vivo bioluminescence imaging was performed at 6 h after the administration. (c) Time course of the induction of the OC-Luc transgene by vitamin D treatment. OC-Luc Tg mice were given a single oral administration of 1 α ,25(OH) 2 D3 (filled circles), 1 α ,25(OH) 2 D2 (shaded circles), or vehicle (open circles). The bioluminescence was analyzed at 0, 6, 9, and 24 h after the administration using Living Image software and represented as the ratio of total flux (photons/second) compared to 0 h. The data shown are means ± SE ( n = 3). Statistical analysis was performed by Student's t -test; * P < 0.05 relative to vehicle.

Article Snippet: Dimethylsulfoxide (DMSO) as the vehicle or 1 nM of previtamin D2 (Sigma-Aldrich Co., St. Louis, MO, USA), previtamin D3 (Sigma-Aldrich), 1 α ,25(OH) 2 D2 (Sigma-Aldrich), and 1 nM 1 α ,25(OH) 2 D3 (Merck KGaA, Darmstadt, Germany) in DMSO (0.01% final DMSO concentration) were added at the same time as the transfection.

Techniques: In Vivo, Expressing, Imaging, Software